EnzyChrom™ Fumarase Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of fumarase enzyme activity.

Key Features
Fast and sensitive. Linear detection range: 0.4 to 70 U/L for 30 min reaction at 37°C.

Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
OD565nm

Samples
Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)

Species
All

Procedure
40 min

Size
100 tests

Detection Limit
0.4 U/L

Shelf Life
6 months

More Details
FUMARASE (or fumarate hydratase) (EC 4.2.1.2) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc. BioAssay Systems' non-radioactive, colorimetric fumarase assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please inquire via email or call 1-510-782-9988 x 2.
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EnzyChrom™ Fumarase Assay Kit
Catalog No: EFMR-100
Price: $459    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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