EnzyChrom™ Fructose Assay Kit

EnzyChrom™ Fructose Assay Kit
Catalog No: EFRU-100
Price: $389    Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment:Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C
Fructose Assay Kit
Protocol SDS


  • For quantitative determination of fructose and evaluation of drug effects on its metabolism.

Key Features

  • Use as little as 20 µL samples. Linear detection range in 96-well plate: 12 to 1000 µM fructose.


  • OD565nm


  • Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc


  • All


  • 70 min


  • 100 tests

Detection Limit

  • 12 µM

Shelf Life

  • 6 months

More Details

  • Fructose (C6H12O6, also called levulose or laevulose), is a monosaccharide found in honey, tree fruits, berries, melons, and some root vegetables along with glucose and galactose. The human body can use fructose for energy, however, too much consumption may lead to high triglycerides. Simple, direct and high-throughput assays for fructose determination find wide applications. BioAssay Systems reagent systems reacts directly and specifically with fructose to form a colored product. Glucose and galactose do not interfere. The color intensity at 565nm is directly proportional to the fructose concentration in the sample.

Do you think it is absolutely necessary to run a standard curve every time you do the assay? If so, why? I have noticed that my standard curves are almost identical every time.

It really depends on you. If you see each time the standard curve is nearly identical, it is not necessary. As a supplier we recommend customers run a standard curve each time with the samples.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Kawabata, K.,et al. (2019). A high-fructose diet induces epithelial barrier dysfunction and exacerbates the severity of dextran sulfate sodium-induced colitis. International Journal of Molecular Medicine, 43(3), 1487-1496. Assay: Fructose in mice feces.

Togo, J., et al. (2019). Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: Liquid or solid. Molecular Metabolism, 27, 22-32. Assay: Fructose in Male C57BL/6J mice serum.

Hoskins, E. C., et al. (2021). Pre-implantation exogenous progesterone and pregnancy in sheep: I. polyamines, nutrient transport, and progestamedins. Journal of Animal Science and Biotechnology, 12(1), 39. Assay: Fructose in Suffolk mature ewes uterine flushings.

Andres-Hernando, A., et al. (n.d.). Vasopressin mediates fructose-induced metabolic syndrome by activating the V1b receptor. JCI Insight, 6(1). Assay: Fructose in mice urine.

Kobayashi, K.,et al. (2019). Large-scale preparation of β-1,2-glucan using quite a small amount of sophorose. Bioscience, Biotechnology, and Biochemistry, 83(10), 1867-1874. Assay: Fructose in Bifidobacterium longum subsp. Longum sucrose phosphorylase.

Montrose, D. C., et al. (2021). Dietary fructose alters the composition, localization, and metabolism of gut microbiota in association with worsening colitis. Cellular and Molecular Gastroenterology and Hepatology, 11(2), 525-550. Assay: Fructose in mice feces.

Sanchez-Lozada, Laura G., et al (2019). Uric acid activates aldose reductase and the polyol pathway for endogenous fructose and fat production causing development of fatty liver in rats. Journal of Biological Chemistry 294.11: 4272-4281. Assay: Fructose in human cells.

Herring, Cassandra Marie (2018). Dietary Arginine Supplementation Enhances Placental Water Transport and Angiogenesis in Gestating Gilts. Diss. Assay: Fructose in gilt plasma.

Milagres, Tamara, et al (2018). Rehydration with fructose worsens dehydration-induced renal damage. BMC nephrology 19.1: 180. Assay: Fructose in mice serum.

Villegas, Leah R., et al (2018). Effects of fructose-containing sweeteners on fructose intestinal, hepatic, and oral bioavailability in dual-catheterized rats. PloS one 13.11: e0207024. Assay: Fructose in rat plasma.

Buntin, Nirunya, et al (2017). An inducible operon is involved in inulin utilization in Lactobacillus plantarum strains, as revealed by comparative proteogenomics and metabolic profiling. Appl. Environ. Microbiol. 83.2: e02402-16. Assay: Fructose in L. plantarum (Bacteria) cells.

Johnson, Richard J (2017). Dehydration as a Cause of Chronic Kidney Disease: Role of Fructokinase. University of Colorado, Denver Aurora United States. Assay: Fructose in mice serum.

Turner, Ryan (2017). Natural sweetener. U.S. Patent Application No. 15/291,710. Assay: Fructose in human serum.

Yau, Adora, et al (2017). The acute effects of simple sugar ingestion on appetite, gut-derived hormone response, and metabolic markers in men. Nutrients 9.2: 135. Assay: Fructose in human serum.

Yau, Adora, et al (2017). The effect of short-term dietary fructose supplementation on gastric emptying rate and gastrointestinal hormone responses in healthy men. Nutrients 9.3: 258. Assay: Fructose in human serum.

Huber, Klaus R., et al (2016). In Vitro Culture of human Brown Adipocytes: Effects of Fructose. International Journal 6.1: 1-8. Assay: Fructose in human cells.

Larson, Jill Marie (2016). Metabolic status of late gestation beef cows and neonatal calves. Diss. University of Missouri--Columbia. Assay: Fructose in calf serum.

Li, Minglan (2016). Developmental programming and reversibility in early life. Diss. ResearchSpace@ Auckland, 2016. Assay: Fructose in rat plasma.

Roncal-Jimenez, Carlos A., et al (2016). Aging-associated renal disease in mice is fructokinase dependent. American Journal of Physiology-Renal Physiology 311.4: F722-F730. Assay: Fructose in mice serum.

Song, Zhilin, et al (2016). Role of fructose and fructokinase in acute dehydration-induced vasopressin gene expression and secretion in mice. Journal of neurophysiology 117.2: 646-654. Assay: Fructose in mice tissues.

Wesseling, Catharina, et al (2016). Kidney function in sugarcane cutters in Nicaragua-A longitudinal study of workers at risk of Mesoamerican nephropathy. Environmental research 147: 125-132. Assay: Fructose in human urine.

Li, M et al (2014). Maternal taurine supplementation attenuates maternal fructose-induced metabolic and inflammatory dysregulation and partially reverses adverse metabolic programming in offspring. Journal of Nutritional Biochemistry 26(3): 267-276. Assay: Fructose in rat plasma.

Roncal Jimenez, CA et al (2014). Fructokinase activity mediates dehydration-induced renal injury. Kidney International 86(2): 294-302. Assay: Fructose in mouse seum.

Sharma, N (2014). Sex differences in renal and metabolic responses to a high-fructose diet in mice. American Journal of Physiology - Renal Physiology 308(5): F4100-10. Assay: Fructose in mouse urine.

Sullivan, JS et al (2014). Oral fructose absorption in obese children with non-alcoholic fatty liver disease. Pediatric Obesity 10(3):188-95. Assay: Fructose in human seum.

Anderstam, B et al (2013). Differences in acute metabolism of fructose between hemodialysis patients and healthy subjects. Scand J Clin Lab Invest. 73(2):154-60. Assay: Fructose in human serum & plasma.

Ishimoto, T et al (2013). High-fat and high-sucrose (western) diet induces steatohepatitis that is dependent on fructokinase. Hepatology 58(5): 1632-1643. Assay: Fructose in mouse serum.

Vickers, M.H., et al. (2011). Maternal fructose intake during pregnancy and lactation alters placental growth and leads to sex-specific changes in fetal and neonatal endocrine function. Endocrinology 152(4):1378-87. Assay: Fructose in rat plasma.

To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.
Buy Now!!!
EnzyChrom™ Fructose Assay Kit
Catalog No: EFRU-100
Price: $389     Out Of Stock
For orders of 10 or more kits, please call +1-510-782-9988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment:Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C
Fructose Assay Kit

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