EnzyChrom™ Fructose Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of fructose and its metabolism.

Key Features
Use as little as 20 μL samples. Linear detection range in 96-well plate: 12 to 1000 μM fructose.

Method
OD565nm

Samples
Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc

Species
All

Procedure
70 min

Size
100 tests

Detection Limit
12 μM

Shelf Life
6 months

More Details
Fructose (C6H12O6, also called levulose or laevulose), is a monosaccharide found in honey, tree fruits, berries, melons, and some root vegetables along with glucose and galactose. The human body can use fructose for energy, however, too much consumption may lead to high triglycerides. Simple, direct and high-throughput assays for fructose determination find wide applications. BioAssay Systems reagent systems reacts directly and specifically with fructose to form a colored product. Glucose and galactose do not interfere. The color intensity at 565nm is directly proportional to the fructose concentration in the sample.

1. Do you think it is absolutely necessary to run a standard curve every time you do the assay? If so, why? I have noticed that my standard curves are almost identical every time.



It really depends on you. If you see each time the standard curve is nearly identical, it is not necessary. As a supplier we recommend customers run a standard curve each time with the samples.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Sanchez-Lozada, Laura G., et al (2019). Uric acid activates aldose reductase and the polyol pathway for endogenous fructose and fat production causing development of fatty liver in rats. Journal of Biological Chemistry 294.11: 4272-4281. Assay: Fructose in human cells.

Herring, Cassandra Marie (2018). Dietary Arginine Supplementation Enhances Placental Water Transport and Angiogenesis in Gestating Gilts. Diss. Assay: Fructose in gilt plasma.

Milagres, Tamara, et al (2018). Rehydration with fructose worsens dehydration-induced renal damage. BMC nephrology 19.1: 180. Assay: Fructose in mice serum.

Villegas, Leah R., et al (2018). Effects of fructose-containing sweeteners on fructose intestinal, hepatic, and oral bioavailability in dual-catheterized rats. PloS one 13.11: e0207024. Assay: Fructose in rat plasma.

Buntin, Nirunya, et al (2017). An inducible operon is involved in inulin utilization in Lactobacillus plantarum strains, as revealed by comparative proteogenomics and metabolic profiling. Appl. Environ. Microbiol. 83.2: e02402-16. Assay: Fructose in L. plantarum (Bacteria) cells.

Johnson, Richard J (2017). Dehydration as a Cause of Chronic Kidney Disease: Role of Fructokinase. University of Colorado, Denver Aurora United States. Assay: Fructose in mice serum.

Turner, Ryan (2017). Natural sweetener. U.S. Patent Application No. 15/291,710. Assay: Fructose in human serum.

Yau, Adora, et al (2017). The acute effects of simple sugar ingestion on appetite, gut-derived hormone response, and metabolic markers in men. Nutrients 9.2: 135. Assay: Fructose in human serum.

Yau, Adora, et al (2017). The effect of short-term dietary fructose supplementation on gastric emptying rate and gastrointestinal hormone responses in healthy men. Nutrients 9.3: 258. Assay: Fructose in human serum.

Huber, Klaus R., et al (2016). In Vitro Culture of human Brown Adipocytes: Effects of Fructose. International Journal 6.1: 1-8. Assay: Fructose in human cells.

Larson, Jill Marie (2016). Metabolic status of late gestation beef cows and neonatal calves. Diss. University of Missouri--Columbia. Assay: Fructose in calf serum.

Li, Minglan (2016). Developmental programming and reversibility in early life. Diss. ResearchSpace@ Auckland, 2016. Assay: Fructose in rat plasma.

Roncal-Jimenez, Carlos A., et al (2016). Aging-associated renal disease in mice is fructokinase dependent. American Journal of Physiology-Renal Physiology 311.4: F722-F730. Assay: Fructose in mice serum.

Song, Zhilin, et al (2016). Role of fructose and fructokinase in acute dehydration-induced vasopressin gene expression and secretion in mice. Journal of neurophysiology 117.2: 646-654. Assay: Fructose in mice tissues.

Wesseling, Catharina, et al (2016). Kidney function in sugarcane cutters in Nicaragua-A longitudinal study of workers at risk of Mesoamerican nephropathy. Environmental research 147: 125-132. Assay: Fructose in human urine.

Li, M et al (2014). Maternal taurine supplementation attenuates maternal fructose-induced metabolic and inflammatory dysregulation and partially reverses adverse metabolic programming in offspring. Journal of Nutritional Biochemistry 26(3): 267-276. Assay: Fructose in rat plasma.

Roncal Jimenez, CA et al (2014). Fructokinase activity mediates dehydration-induced renal injury. Kidney International 86(2): 294-302. Assay: Fructose in mouse seum.

Sharma, N (2014). Sex differences in renal and metabolic responses to a high-fructose diet in mice. American Journal of Physiology - Renal Physiology 308(5): F4100-10. Assay: Fructose in mouse urine.

Sullivan, JS et al (2014). Oral fructose absorption in obese children with non-alcoholic fatty liver disease. Pediatric Obesity 10(3):188-95. Assay: Fructose in human seum.

Anderstam, B et al (2013). Differences in acute metabolism of fructose between hemodialysis patients and healthy subjects. Scand J Clin Lab Invest. 73(2):154-60. Assay: Fructose in human serum & plasma.

Ishimoto, T et al (2013). High-fat and high-sucrose (western) diet induces steatohepatitis that is dependent on fructokinase. Hepatology 58(5): 1632-1643. Assay: Fructose in mouse serum.

Vickers, M.H., et al. (2011). Maternal fructose intake during pregnancy and lactation alters placental growth and leads to sex-specific changes in fetal and neonatal endocrine function. Endocrinology 152(4):1378-87. Assay: Fructose in rat plasma.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyChrom™ Fructose Assay Kit
Catalog No: EFRU-100
Price: $339    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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