EnzyChrom™ Fructose Assay Kit
ProtocolSDS


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Application
For quantitative determination of fructose and its metabolism.

Key Features
Use as little as 20 μL samples. Linear detection range in 96-well plate: 12 to 1000 μM fructose.

Method
OD565nm

Samples
Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc

Species
All

Procedure
70 min

Size
100 tests

Detection Limit
12 μM

Shelf Life
6 months

More Details
Fructose (C6H12O6, also called levulose or laevulose), is a monosaccharide found in honey, tree fruits, berries, melons, and some root vegetables along with glucose and galactose. The human body can use fructose for energy, however, too much consumption may lead to high triglycerides. Simple, direct and high-throughput assays for fructose determination find wide applications. BioAssay Systems reagent systems reacts directly and specifically with fructose to form a colored product. Glucose and galactose do not interfere. The color intensity at 565nm is directly proportional to the fructose concentration in the sample.

1. Do you think it is absolutely necessary to run a standard curve every time you do the assay? If so, why? I have noticed that my standard curves are almost identical every time.



It really depends on you. If you see each time the standard curve is nearly identical, it is not necessary. As a supplier we recommend customers run a standard curve each time with the samples.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Anderstam, B et al (2013). Differences in acute metabolism of fructose between hemodialysis patients and healthy subjects. Scand J Clin Lab Invest. 73(2):154-60. Assay: Fructose in human serum & plasma (Pubmed).

2. Ishimoto, Tet al (2013). High-fat and high-sucrose (western) diet induces steatohepatitis that is dependent on fructokinase. Hepatology. 58(5):1632-43. Assay: Fructose in mice NA (Pubmed).

3. Vickers, MH et al (2011). Maternal fructose intake during pregnancy and lactation alters placental growth and leads to sex-specific changes in fetal and neonatal endocrine function. Endocrinology 152(4):1378-87. Assay: Fructose in Rat Plasma (Pubmed).

4. Roncal Jimenez, CA et al (2014). Fructokinase activity mediates dehydration-induced renal injury. Kidney International 86(2): 294-302. Assay: Fructose in Mouse seum (Pubmed).

5. Sullivan, JS et al (2014). Oral fructose absorption in obese children with non-alcoholic fatty liver disease. Pediatric Obesity. Assay: Fructose in Human seum (Pubmed).

6. Li, M et al (2014). Maternal taurine supplementation attenuates maternal fructose-induced metabolic and inflammatory dysregulation and partially reverses adverse metabolic programming in offspring. Journal of Nutritional Biochemistry 26(3): 297-276. Assay: Fructose in Rat plasma (Pubmed).

7. Sharma, N (2014). Sex differences in renal and metabolic responses to a high-fructose diet in mice. American Journal of Physiology - Renal Physiology 308(5): F4100-10. Assay: Fructose in Mouse urine (Pubmed).
To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
Buy Now!!!
EnzyChrom™ Fructose Assay Kit
Catalog No: EFRU-100
Price: $339    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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