EnzyChrom™ Ethanol Assay Kit
Datasheet PDF  MSDS/Bulk Order

For quantitative determination of ethanol and evaluation of drug effects on alcohol metabolism.

Key Features
Sensitive and accurate. Detection limit 0.0008 vol % (140 μM or 8 ppm), linearity up to 0.1% ethanol in 96-well plate assay.

Convenient. The procedure involves adding working reagent, incubating for 30 min and stopping reaction. No 37°C heater is needed.

High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

OD565nm (Enzymatic)

Serum, plasma, urine, saliva samples etc


30 min

100 tests

Detection Limit

Shelf Life
6 months

More Details
Alcoholic drinks are among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C2H5OH) has applications in basic research, drug discovery, clinic studies and in the alcoholic industry. Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable. BioAssay Systems’ EnzyChrom™ ethanol assay kit is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565 nm, is proportionate to the ethanol concentration in the sample.

1. Does the assay react with methanol?

No, the assay does essentially not cross-react with methanol. Only at high concentrations (more than 100 mM methanol) will there be any significant nonspecific signal.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Jeong WI et al (2008). Paracrine activation of hepatic CB1 receptors by stellate cell-derived endocannabinoids mediates alcoholic fatty liver. Cell Metab. 7(3):227-35. Assay: Ethanol in Mouse serum (Pubmed).

2. Ou XM et al (2010). A novel role for glyceraldehyde-3-phosphate dehydrogenase and monoamine oxidase B cascade in ethanol-induced cellular damage. Biol Psychiatry 67(9):855-63. Assay: Ethanol in Human neuroblastoma cell (Pubmed).

3. Suwannarangsee S et al (2010). Characterization of alcohol dehydrogenase 1 of the thermotolerant methylotrophic yeast Hansenula polymorpha. Appl Microbiol Biotechnol. 88(2):497-507. Assay: Ethanol in Yeast cell (Pubmed).

4. Magne L et al (2011). ATF4 and the integrated stress response are induced by ethanol and cytochrome P450 2E1 in human hepatocytes. J Hepatol. 54(4):729-37. Assay: Ethanol in Human hepatocytes (Pubmed).

5. Echevarria DJ et al (2011). Alcohol-induced behavior change in zebrafish models. Rev Neurosci. 22(1):85-93. Assay: Ethanol in Zebrafish blood (Pubmed).

6. Echevarria,DJ and Hammack,CH (2010). Does acute alcohol exposure modulate aggressive behaviors in the zebrafish (Danio rerio), or is the bark worse than the bite. ijcp 23:62-69. Assay: Ethanol in Zebrafish blood (Pubmed).

7. Pariset, JJ et al (2009). Alcohol Dose-Dependently Enhances 3 alpha-Androstanediol Formation in Frontal Cortex of Male Rats Concomitant with Aggression. Open Neuropsychopharmacology 2:1-10. Assay: Ethanol in Rat blood (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ Ethanol Assay Kit
Catalog No: ECET-100
Price: $299    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C

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