QuantiFluo™ DNA Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of DNA.

Key Features
Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay.

Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day.

Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay.

Method
FL340/450nm

Samples
Plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices

Species
All

Procedure
5 min

Size
250 tests

Detection Limit
100 ng/mL

Shelf Life
12 months

More Details
DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling. Simple, direct and automation-ready procedures for measuring DNA concentration are very desirable. BioAssay Systems’ QuantiFluo™ DNA assay kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at λex/em = 350/450nm, is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

1. Can you guarrantee, that your kit enables an accurate estimation of cDNA concentration in RT reaction mixture, where high excess of total RNA is present? Is it a SYBR Green based method?



We are using Hoechst dye based DNA assay. It is very specific for DNA and not RNA. However, as we do not know the RNA content in your sample, we can not judge if it would meet your needs.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Waheed MM et al (2011). Some Biochemical Characteristics and Preservation of Epididymal Camel Spermatozoa (Camelus dromedarius). Theriogenology 2011 Jul 13. Assay: DNA in Human cell line (Pubmed).

2. Lai, M et al (2007). 2007 AAPS Annual Meeting & Exposition. Development of an In-Vitro Screening Method to Determine Enzymatic Release Rates from a Homologous Series of Novel SN-38 Conjugates. Sonus Pharmaceuticals. Assay: DNA in Human cells, stem cells (Pubmed).

3. Bhavnani BR et al (2008). Structure activity relationships and differential interactions and functional activity of various equine estrogens mediated via estrogen receptors (ERs) ERalpha and ERbeta. Endocrinology. 149(10):4857-70. Assay: DNA in Rat microglial cells (Pubmed).

4. Mayer-Wagner S et al (2010). Membrane-based cultures generate scaffold-free neocartilage in vitro: influence of growth factors. Tissue Eng Part A. 16(2):513-21. Assay: DNA in Human stem cell (Pubmed).

5. Yasuda, N (2007). EFFECTS OF LONG-TERM STRENUOUS EXERCISE ON OXIDATIVE DNA DAMAGE AND PROTEINURIA IN HUMANS. Ph.D. Thesis. Assay: DNA in Rat primary glial culture (Pubmed).

To find more recent publications, please click here.
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QuantiFluo™ DNA Assay Kit
Catalog No: QFDN-250
Price: $259    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: RT
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: 4°C



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