EnzyFluo™ D-Lactate Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

Key Features
Sensitive and accurate. Detection limit of 1 μM and linearity up to 50 μM D-lactate in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay.

High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method
FL530/585 nm

Samples
Serum, plasma, cell culture media etc

Species
All

Procedure
60 min

Size
100 tests

Detection Limit
1 μM

Shelf Life
6 months

More Details
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-Lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems EnzyFluo™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the lactate concentration in the sample.
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For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Palomino, Nahui, et al (2017). Vaginal Lactobacillus inhibits HIV-1 replication in human tissues ex vivo. Frontiers in microbiology 8: 906. Assay: D-Lactate in Lactobacillus culture.

Saville, Renee M., et al (2016). Compositions and methods for biological production of lactate from C1 compounds using lactate dehydrogenase transformants. U.S. Patent Application No. 14/898,948. Assay: D-Lactate in microorganism culture.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyFluo™ D-Lactate Assay Kit
Catalog No: EFDLC-100
Price: $349    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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