EnzyChrom™ D-Amino Acid Assay Kit
Protocol SDS   Printed Protocols and SDSs are not provided with kits.

Application
•   Quantitative determination of D-Amino Acids in biological and other samples.

Key Features
•   Fast and sensitive. Linear detection range: 0.86 to 500 µM (colorimetric assay) and 0.18 to 50 µM (fluorimetric assay) for 60 min reaction.

•   Convenient. The procedure involves adding a single working reagent and reading after 60 minutes.

•   High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day.

Method
•   OD570nm or FL530/585nm

Samples
•   Tissue, milk, and other biological samples.

Species
•   All

Procedure
•   60 min

Size
•   100 tests

Shelf Life
•   6 months

More Details
•   D-AMINO-ACIDS are not as widespread as their enantiomeric counterparts in proteins but they can be found in organisms ranging from bacteria (cell walls and antibiotics) to mammals (central nervous systems). The presence of D-amino acids in food is also of considerable interest. Racemization of L-amino acids during food processing may affect food quality and nutritional value.

BioAssay Systems' D-amino acid assay uses an enzyme-catalyzed oxidation of D-amino acids to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the D-amino acid concentration in the sample.

1. Will this kit detect L-amino acids?



The DAA enzyme is specific to D-amino acids only.

2. What samples have you tested?



This kit has been tested on rat brain tissue, mouse brain tissue, cerebrospinal fluid, and dairy products like whole milk.

3. Can I store unused reagents for future use?



Yes, unused reagents can be stored according to the assay protocol.

4. How do you use this kit on cerebrospinal fluid?



CSF can be assayed directly and an internal standard should be used.
Samples will need three separate reactions:
1) sample plus standard
2) sample alone
3) sample blank.
For the internal standard prepare 200 µL 1250 µM standard by mixing 125 µL 2 mM Standard and 75 µL dH2O.
For the sample plus standard well, add 5 µL 1250 µM standard and 20 µL sample.
For the sample and sample blank wells, add 5 µL dH2O and 20 µL sample.
Prepare enough Working Reagent (WR) for all assay wells by mixing, for each well, 85 µL Assay Buffer, 1 µL DAA Enzyme, 1 µL HRP Enzyme, 1 µL Dye Reagent. Prepare a WR without the DAA Enzyme for the sample blank well(s).
Add 80 µL of the WR to each well. Tap plate briefly to mix.
Incubate at room temperature for 60 min. Use a plate reader to read OD570nm.
The sample D-amino acid concentration is computed as follows:
EDAA Internal Standard Equation
where RSAMPLE, RBLANK, and RSTANDARD are the OD or fluorescence values of the Sample, Sample Blank, and the Sample plus Standard respectively. n is the sample dilution factor. Note: The volume of the internal standard is 4× lower than the sample volume; thus, the internal standard concentration should be divided by 4.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
No citations for this new product. Please check back later.
You may click here to check if citations are available, but are not listed here yet.
Please inquire or request assay service or call 1-510-782-9988 x 2.
Buy Now!!!
EnzyChrom™ D-Amino Acid Assay Kit
Catalog No: EDAA-100
Price: $399    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

Related Products


Why BioAssay Systems?

Expert Technical Support
Technical support provided by the very scientists that develop the assays.

Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market

Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.

Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.


BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers