QuantiChrom™ Creatinine Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism.

Key Features
Sensitive and accurate. Use 30 μL samples. Detection limit 0.10 mg/dL (8 μM) creatinine in 96-well plate assay.

Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day.

Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet.

Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples.

Method
OD510nm

Samples
Urine, serum, plasma and biological preparations

Species
All

Procedure
20 min

Size
500 tests

Detection Limit
0.1 mg/dL (8 μM)

Shelf Life
12 months

More Details
Creatinine is synthesized in the body at a fairly constant rate from creatine, which is produced during muscle contractions from creatine phosphate. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, whereas the creatinine clearance rate and hence the urine levels are diminished. Creatinine test is most widely used to assess kidney function. Simple, direct and automation-ready procedures for measuring creatinine concentration in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems creatinine assay kit is designed to measure creatinine directly in biological samples without any pretreatment. The improved Jaffe method utilizes picrate that forms a red colored complex with creatinine. The intensity of the color, measured at 510nm, is directly proportional to creatinine concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw sample.

1. If I understand correctly, there is no need to perform a full creatinine standard calibration curve. The 2 mg/dL point will suffice. Is this right?



Yes, the reaction is linear from 0 up to 50 mg/dL and a single 2mg/dL creatinine standard is sufficient.

2. Is this assay compatible with plasma prepared using EDTA?



Yes, EDTA plasma is compatible with the assay.

3. I have read the specification sheet and I didn't find details about the temperature of the reaction. Due to it is a kinetic reaction, I am wondering if the 5 minutes incubation should be done at 37°C or room temperature is fine. Do you have some additional information about this?



We normally perform the assay at room temperature but you can run it also at 37°C if this is more convenient. The absorbance values will be higher at 37°C, but because you run the standard and the samples under the same conditions, a change in temperature has no effect on the accuracy of the measurement. It is more important to keep the time between adding the reagent and the OD measurement about the same for all samples, e.g. by using a multichannel pipettor.

4. I would like to know if the QuantiChrom Creatinine Assay kit can be used for urine samples from rat and mouse.



Yes, this assay can be used for urine samples from any species including rat and mouse.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Natarajan, M., Habib, S. L., Reddick, R. L., Delma, C. R., Manickam, K., Prihoda, T. J. & Mohan, S. (2019). Endothelial cell-specific overexpression of endothelial nitric oxide synthase in Ins2Akita mice exacerbates diabetic nephropathy. Journal of Diabetes and its Complications, 33(1), 23-32. Assay: Creatinine in mouse urine.

Patel, S., Dhande, I., Gray, E. A., Ali, Q., & Hussain, T. (2019). Prevention of lipopolysaccharide-induced CD11b+ immune cell infiltration in the kidney: role of AT2 receptors. Bioscience reports, 39(5), BSR20190429. Assay: Creatinine in mice plasma/urine.

Vorland, C. J., Lachcik, P. J., Swallow, E. A., Metzger, C. E., Allen, M. R., Chen, N. X. & Gallant, K. M. H. (2019). Effect of ovariectomy on the progression of chronic kidney disease-mineral bone disorder (CKD-MBD) in female Cy/+ rats. Scientific reports, 9(1), 7936. Assay: Creatinine in rats plasma.

Zhu, J., Wang, Q., Li, C., Lu, Y., Hu, H., Qin, B. & Wang, S. (2019). Inhibiting inflammation and modulating oxidative stress in oxalate-induced nephrolithiasis with the Nrf2 activator dimethyl fumarate. Free Radical Biology and Medicine, 134, 9-22. Assay: Creatinine in Sprague Dewley rats plasma.

Jing, W., Jabbari, B., & Vaziri, N. D. (2018). Uremia induces upregulation of cerebral tissue oxidative/inflammatory cascade, down-regulation of nrf2 pathway and disruption of blood brain barrier. American journal of translational research, 10(7), 2137-2147. Assay: Creatinine in rats urine.

Qiu, S., Chen, X., Pang, Y., & Zhang, Z. (2018). Lipocalin-2 protects against renal ischemia/reperfusion injury in mice through autophagy activation mediated by HIF1alpha and NF-kb crosstalk. Biomedicine & Pharmacotherapy, 108, 244-253. Assay: Creatinine in mice serum.

Shen, W., Jiang, X. X., Li, Y. W., & He, Q. (2018). Mitochondria-mediated disturbance of fatty acid metabolism in proximal tubule epithelial cells leads to renal interstitial fibrosis. Eur Rev Med Pharmacol Sci, 22(3), 810-819. Assay: Creatinine in mice kidney cells.

Yin, D. D., Luo, J. H., Zhao, Z. Y., Liao, Y. J., & Li, Y. (2018). Tranilast prevents renal interstitial fibrosis by blocking mast cell infiltration in a rat model of diabetic kidney disease. Molecular medicine reports, 17(5), 7356-7364. Assay: Creatinine in rats kidney cells.

Choi, J. Y., Seth, A., Kashgarian, M., Terrillon, S., Fung, E., Huang, L. & Craft, J. (2017). Disruption of pathogenic cellular networks by IL-21 blockade leads to disease amelioration in murine lupus. The Journal of Immunology, 198(7), 2578-2588. Assay: Creatinine in rats serum.

Chuang, P. Y., Cai, W., Li, X., Fang, L., Xu, J., Yacoub, R. & Lee, K. (2017). Reduction in podocyte SIRT1 accelerates kidney injury in aging mice. American Journal of Physiology-Renal Physiology, 313(3), F621-F628. Assay: Creatinine in mice urine.

Crislip, G. R., O'Connor, P. M., Wei, Q., & Sullivan, J. C. (2017). Vasa recta pericyte density is negatively associated with vascular congestion in the renal medulla following ischemia reperfusion in rats. American Journal of Physiology-Renal Physiology, 313(5), F1097-F1105. Assay: Creatinine in rats arterial blood.

Dutta, R. K., Kondeti, V. K., Sharma, I., Chandel, N. S., Quaggin, S. E., & Kanwar, Y. S. (2017). Beneficial effects of myo-inositol oxygenase deficiency in cisplatin-induced AKI. Journal of the American Society of Nephrology, 28(5), 1421-1436. Assay: Creatinine in mice blood.

Fan, Y., Zhang, J., Xiao, W., Lee, K., Li, Z., Wen, J. & Sheng, X. (2017). Rtn1a-mediated endoplasmic reticulum stress in podocyte injury and diabetic nephropathy. Scientific reports, 7(1), 323. Assay: Creatinine in mice urine.

Jing, W., Vaziri, N. D., Nunes, A., Suematsu, Y., Farzaneh, T., Khazaeli, M., & Moradi, H. (2017). LCZ696 (Sacubitril/valsartan) ameliorates oxidative stress, inflammation, fibrosis and improves renal function beyond angiotensin receptor blockade in CKD. American journal of translational research, 9(12), 5473-5484. Assay: Creatinine in sprague dewley rats serum.

Kumar, P., Gogulamudi, V. R., Periasamy, R., Raghavaraju, G., Subramanian, U., & Pandey, K. N. (2017). Inhibition of HDAC enhances STAT acetylation, blocks NF-kB, and suppresses the renal inflammation and fibrosis in Npr1 haplotype male mice. American Journal of Physiology-Renal Physiology, 313(3), F781-F795. Assay: Creatinine in mice urine.

Liao, H. K., Hatanaka, F., Araoka, T., Reddy, P., Wu, M. Z., Sui, Y. & Guillen, P. (2017). In vivo target gene activation via CRISPR/Cas9-mediated trans-epigenetic modulation. Cell, 171(7), 1495-1507. Assay: Creatinine in mice urine.

West, D., Abou Sawan, S., Mazzulla, M., Williamson, E., & Moore, D. (2017). Whey protein supplementation enhances whole body protein metabolism and performance recovery after resistance exercise: A double-blind crossover study. Nutrients, 9(7), 735. Assay: Creatinine in human urine.

Xiao, L., Xu, X., Zhang, F., Wang, M., Xu, Y., Tang, D. & He, L. (2017). The mitochondria-targeted antioxidant MitoQ ameliorated tubular injury mediated by mitophagy in diabetic kidney disease via Nrf2/PINK1. Redox biology, 11, 297-311. Assay: Creatinine in mice blood/urine.

Fujino, T., & Hasebe, N. (2016). Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy. BMC nephrology, 17(1), 179. Assay: Creatinine in mouse cells.

Harley, K. G., Kogut, K., Madrigal, D. S., Cardenas, M., Vera, I. A., Meza-Alfaro, G. & Eskenazi, B. (2016). Reducing phthalate, paraben, and phenol exposure from personal care products in adolescent girls: findings from the HERMOSA intervention study. Environmental health perspectives, 124(10), 1600-1607. Assay: Creatinine in human urine.

Kim, J. (2016). Poly (ADP-ribose) polymerase activation induces high mobility group box 1 release from proximal tubular cells during cisplatin nephrotoxicity. Physiological research 65(2): 333-340. Assay: Creatinine in mice blood from kidney.

Min, D., Brooks, B., Wong, J., Aamidor, S., Seehoo, R., Sutanto, S. & McLennan, S. V. (2016). Monocyte CD163 is altered in association with diabetic complications: possible protective role. Journal of leukocyte biology, 100(6), 1375-1383. Assay: Creatinine in mice plasma.

Pai, C. H., Yen, C. T., Chen, C. P., Yu, I. S., Lin, S. W., & Lin, S. R. (2016). Lack of Thromboxane synthase prevents hypertension and fetal growth restriction after high salt treatment during pregnancy. PloS one, 11(3), e0151617. Assay: Creatinine in mice urine.

Zheng, S., Coventry, S., Cai, L., Powell, D. W., Jala, V. R., Haribabu, B., & Epstein, P. N. (2016). Renal protection by genetic deletion of the atypical chemokine receptor ACKR2 in diabetic OVE mice. Journal of diabetes research, 2016:5362506. Assay: Creatinine in mice serum.

Zhong, F., Mallipattu, S. K., Estrada, C., Menon, M., Salem, F., Jain, M. K. & He, J. C. (2016). Reduced kruppel-like factor 2 aggravates glomerular endothelial cell injury and kidney disease in mice with unilateral nephrectomy. The American journal of pathology, 186(8), 2021-2031. Assay: Creatinine in Kfl2 Mice urine.

Zhou, X., Chen, K., Wang, Y., Schuman, M., Lei, H., & Sun, Z. (2016). Antiaging gene Klotho regulates adrenal CYP11B2 expression and aldosterone synthesis. Journal of the American Society of Nephrology, 27(6), 1765-1776. Assay: Creatinine in mice urine.

Wang J, et al (2011). Elevated oxidative damage in kitchen workers in Chinese restaurants. J Occup Health 53(5):327-33. Assay: Creatinine in human urine.

Abrass CK,et al (2010). Laminin alpha4-null mutant mice develop chronic kidney disease with persistent overexpression of platelet-derived growth factor. Am J Pathol. 176(2):839-49. Assay: Creatinine in mice urine.

Hauser PV, et al (2010). Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery. Am J Pathol. 177(4):2011-21. Assay: Creatinine in mice serum.

Jaruga, P. and Dizdaroglu, M (2010). Identification and quantification of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines in human urine as putative biomarkers of oxidatively induced damage to DNA. BBRC 397(1): 48-52. Assay: Creatinine in human urine.

Kim JW, et al (2010). Increased urinary lipocalin-2 reflects matrix metalloproteinase-9 activity in chronic hepatitis C with hepatic fibrosis. Tohoku J Exp Med. 222(4):319-27. Assay: Creatinine in human urine.

Sahan-Firat, S et al (2010). 2,3',4,5'-Tetramethoxystilbene prevents deoxycorticosterone-salt-induced hypertension: contribution of cytochrome P-450 1B1. Am J Physiol Heart Circ Physiol 299(6):H1891-901. Assay: Creatinine in rat serum.

Tang TC,et al (2010). Impact of metronomic UFT/cyclophosphamide chemotherapy and antiangiogenic drug assessed in a new preclinical model of locally advanced orthotopic hepatocellular carcinoma. Neoplasia 12(3):264-74. Assay: Creatinine in mice urine.

Xu J, et al (2010). FVB mouse genotype confers susceptibility to OVE26 diabetic albuminuria. Am J Physiol Renal Physiol. 299(3):F487-94. Assay: Creatinine in mouse serum.

Andonegui G, et al (2009). Characterization of S. pneumoniae pneumonia-induced multiple organ dysfunction syndrome: an experimental mouse model of gram-positive sepsis. Shock. 31(4):423-8. Assay: Creatinine in mouse plasma.

Grimm, PR et al (2009). Hypertension of Kcnmb1-/- is linked to deficient K secretion and aldosteronism. PNAS 106(28):11800-5. Assay: Creatinine in mouse serum.

Zafar I, et al (2009). Long-term rapamycin therapy in the Han:SPRD rat model of polycystic kidney disease (PKD). Nephrol Dial Transplant. 24(8):2349-53. Assay: Creatinine in rat serum.

He Z, et al (2008). Interleukin-18 binding protein transgenic mice are protected against ischemic acute kidney injury. Am J Physiol Renal Physiol. 295(5):F1414-21. Assay: Creatinine in mice serum.

Gilliam-Davis S, et al (2007). Long-term AT1 receptor blockade improves metabolic function and provides renoprotection in Fischer-344 rats. Am J Physiol Heart Circ Physiol. 293(3):H1327-33. Assay: Creatinine in rat serum.

Lorch, G et al (2007). Inhibition of epidermal growth factor receptor signalling reduces hypercalcaemia induced by human lung squamous-cell carcinoma in athymic mice. Br J Cancer 97(2):183-93. Assay: Creatinine in mouse urine.

Socha MJ, et al (2007). Secreted protein acidic and rich in cysteine deficiency ameliorates renal inflammation and fibrosis in angiotensin hypertension. Am J Pathol. 171(4):1104-12. Assay: Creatinine in mice urine.

Stanic, AK et al (2006). Immune dysregulation accelerates atherosclerosis and modulates plaque composition in systemic lupus erythematosus. PNAS 103(18):7018-23. Assay: Creatinine in mouse serum.

Zhang SX, et al (2006). Therapeutic potential of angiostatin in diabetic nephropathy. J Am Soc Nephrol. 17(2):475-86. Assay: Creatinine in rat urine.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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QuantiChrom™ Creatinine Assay Kit
Catalog No: DICT-500
Price: $319    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4, -20°C

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