EnzyChrom™ Creatine Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of creatine and evaluation of drug effects on creatine metabolism.

Key Features
High sensitivity and wide linear range. Use 10 μL sample. Linear detection range 4 to 1000 μM (colorimetric) or 0.5 to 50 μM (fluorimetric).

Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of creatine within 30 minutes.

Method
OD570nm, or FL530/585nm

Samples
Serum, plasma, urine, saliva etc

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
4 μM

Shelf Life
12 months

More Details
CREATINE is present in vertebrates and helps to supply energy to muscle. In humans and animals, approximately half of creatine originates from food (mainly from fresh meat). Creatine supplementation has been investigated as a possible therapeutic approach for the treatment of muscular, neuromuscular, neurological and neurodegenerative diseases. Simple, direct and automation-ready procedures for measuring creatine are popular in research and drug discovery. BioAssay Systems’ creatine assay is based on enzymatic reactions leading to formation of a pink colored product. The optical density at 570 nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to the creatine concentration in the sample.

1. Can this kit be used to measure creatine concentrations in cells?



Yes. To determine intracellular creatine concentrations, harvest cells (0.1-2 million cells per assay) in a 1.5mL tube, centrifuge 1-2 min at 3,000 rpm on a table centrifuge. Remove culture medium, wash cells quickly with cold PBS. Immediately remove any PBS. Prepare Working Reagent. Add 120 µL WR directly to the cell pellet and 80uL WR to standards in 96-wells. Vortex cell sample tube 1 min, incubate 30 min at room temp. Centrifuge 5 min at 14,000 rpm. Transfer 100 µL supernatant to 96-well. Read OD570nm on a plate reader.

Notes: 1. cell number. It is prudent to run several doses of cells, e.g. 0.1, 1, 2x106 cells to determine optimal cell number to be used in subsequent assays.
2. Working Reagent should contain a lysis reagent. BioAssay Systems will supply this reagent upon request.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Schoborg, J. A., et al (2014). Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis. Biotechnology Journal.10.1002/biot.201300383 Assay: Creatine in yeast protein (CFPS -cell-free protein synthesis) (Pubmed).

2. Schoborg, JA et al (2014). Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis. Biotechnology Journal 9(5): 630-640. Assay: Creatine in Yeast cells (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ Creatine Assay Kit
Catalog No: ECRT-100
Price: $319    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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