EnzyChrom™ Coenzyme A Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism.

Key Features
Sensitive. Use 10 μL samples. Linear detection range: colorimetric assay 5 - 1000 μM, fluorimetric assay 3 - 100 μM CoA.

Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day.

Method
OD570nm, or FL530/585nm

Samples
Biological

Species
All

Procedure
60 min

Size
100 tests

Detection Limit
3 μM

Shelf Life
6 months

More Details
Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of CoA’s most crucial roles is the carrying and transferring of acyl groups. BioAssay Systems method provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the CoA concentration in the sample.
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Takahashi, Takayuki, Yukitoshi Mine, and Tadashi Okamoto (2018). 2, 3-Dimethoxy-5-methyl-p-benzoquinone (Coenzyme Q0) Disrupts Carbohydrate Metabolism of HeLa Cells by Adduct Formation with Intracellular Free Sulfhydryl-Groups, and Induces ATP Depletion and Necrosis. Biological and Pharmaceutical Bulletin 41.12: 1809-1817. Assay: Coenzyme A in human cells.

To find more recent publications, please click here.
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EnzyChrom™ Coenzyme A Assay Kit
Catalog No: ECOA-100
Price: $339    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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