EnzyChrom™ Choline Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of choline and evaluation of drug effects on choline metabolism.

Key Features
Use 20 μL samples. Linear detection range: colorimetric assay 1 to 100 μM, fluorimetric assay 0.2 to 10 μM choline.

Method
OD570nm, or FL530/585nm

Samples
Serum, plasma, urine, saliva, milk, tissue, cells etc

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
OD, FL: 1, 0.2 μM

Shelf Life
6 months

More Details
CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. BioAssay Systems method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to βine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.

1. What are known interferences?



Chondroitin sulfate C (chondroitin-6-sulfate) appears to interfere with the assay; creating false positive results.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Ballester-Lozano, GF et al (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Choline in fish plasma.

Benedito-Palos, L et al (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture 454: 8-18. Assay: Choline in fish plasma.

Ballester-Lozano, GF et al (2014). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Choline in fish plasma.

Strilakou, AA et al (2014). Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats. Canadian Journal of Physiology and Pharmacology 92(1): 78-84. Assay: Choline in rat serum.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
Buy Now!!!
EnzyChrom™ Choline Assay Kit
Catalog No: ECHO-100
Price: $349    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

Related Products


Why BioAssay Systems?

Expert Technical Support
Technical support provided by the very scientists that develop the assays.

Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market

Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.

Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.


BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers