EnzyChrom™ Choline Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of choline and evaluation of drug effects on choline metabolism.

Key Features
Use 20 μL samples. Linear detection range: colorimetric assay 1 to 100 μM, fluorimetric assay 0.2 to 10 μM choline.

Method
OD570nm, or FL530/585nm

Samples
Serum, plasma, urine, saliva, milk, tissue, cells etc

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
OD, FL: 1, 0.2 μM

Shelf Life
6 months

More Details
CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. BioAssay Systems’ method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to βine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.

1. What are known interferences?



Chondroitin sulfate C (chondroitin-6-sulfate) appears to interfere with the assay; creating false positive results.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Ballester-Lozano, GF et al (2014). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. British Journal of Nutrition 114(5): 713-726. Assay: Plasma in Fish.

2. Strilakou, AA et al (2014). Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats. Canadian Journal of Physiology and Pharmacology 92(1): 78-84. Assay: Serum in Rats (Pubmed).

3. Wei, J et al (2014). Soluble Expression and Purification of the Catalytic Domain of Human Vascular Endothelial Growth Factor Receptor 2 in Escherichia coli. Journal of Microbiology and Biotechnology 25(8): 1227-33. Assay: cells in e. coli (Pubmed).

4. Benedito-Palos, L et al (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture 454: 8-18.

To find more recent publications, please click here.
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EnzyChrom™ Choline Assay Kit
Catalog No: ECHO-100
Price: $329    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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