EnzyChrom™ Catalase Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of catalase activity and evaluation of drug effects on catalase activity.

Key Features
Sensitive and accurate. Use 10 μL sample. Linear detection range 0.2 to 5 U/L catalase activity.

Simple and Convenient. The procedure involves adding a Substrate to sample, incubation for 30 min, followed by a Detection Reagent and reading the optical density or fluorescence intensity.

Method
OD570nm, or FL530/585nm

Samples
Serum, plasma, urine, saliva, cell culture etc

Species
All

Procedure
40 min

Size
100 tests

Detection Limit
0.2 U/L

Shelf Life
6 months

More Details
CATALASE (EC 1.11.1.6), is an ubiquitous antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen. By preventing excessive H2O2 build up, catalase allows important cellular processes which produce H2O2 as a byproduct to occur safely. Deficiency in catalase activity has been associated with grey hair and peroxisomal disorder acatalasia. Simple, direct and high-throughput assays for catalase activity find wide applications. BioAssay Systems improved assay directly measures catalase degradation of H2O2 using a redox dye. The change in color intensity at 570nm or fluorescence intensity (λex/em = 530/585nm) is directly proportional to the catalase activity in the sample.

1. Regarding the technique used in the DPOD-100 peroxidase assay and the ECAT-100 catalase assay that seems to be really similar, how will we be sure that one of them will measure the catalase activity and the other the peroxidase?



The DPOD assay measures the conversion of the dye reagent and hydrogen peroxide, which are provided in large excess as substrates for cellular peroxidases. Endogenous catalase activity does not interfere with the assay, because it does not react with the dye reagent, hydrogen peroxide is in large excess, and the reaction is short (10 minutes), leaving the H2O2 concentration practically unchanged.

The ECAT assays measures catalase activity by first incubating much lower concentrations of hydrogen peroxide with the samples for 30 minutes. Then the decrease in hydrogen peroxide is measured by adding the dye reagent and HRP in excess.

2. You will have 100 µL of each standard when you have pipetted all solutions for the standard curve. How stable are the 100 µl standards? Can they be used the next day to make another standard curve or can the values from the already made standard curve be used when you have other samples the next day?



The H2O2 standards are stable for an hour. Therefore assays should be run as soon as possible with the samples and standards. It is recommended that customers run standard curve in each experiment.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Chiu CC et al (2012). Beneficial Effects of Ocimum gratissimum Aqueous Extract on Rats with CCl(4)-Induced Acute Liver Injury. Evid Based Complement Alternat Med 2012:736752. Assay: Catalase in Rat Serum (Pubmed).

2. Zhu T et al (2012) Effects of the iron-chelating agent deferoxamine on triethylene glycol dimethacrylate, 2-hydroxylethyl methacrylate, hydrogen peroxide-induced cytotoxicity. J Biomed Mater Res B Appl Biomater 100(1):197-205. Assay: Catalase in Human Dental pulp cells (Pubmed).

3. Hadzi-Petrushev N et al (2011) L-2-oxothiazolidine-4-carboxylate influence on age- and heat exposure-dependent redox changes in rat’s blood plasma. J Physiol Sci 61(5):437-442. Assay: Catalase in rat plasma (Pubmed).

4. Hadzi-Petrushev N et al (2012). L-2-oxothiazolidine-4-carboxylate influence on age- and heat exposure-dependent peroxidation in rat’s liver and kidney. J Therm Biol 37(5):361-365. Assay: Catalase in Rat Kidney (Pubmed).

5. Labib, HM et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Aust J Basic Appl Sci 4(8): 3553-3558. Assay: Catalase in Human Blood (Pubmed).

To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

- Fast turnaround
- Quality data
- Low cost

Please inquire via email or call 1-510-782-9988 x 2.
Buy Now!!!
EnzyChrom™ Catalase Assay Kit
Catalog No: ECAT-100
Price: $259    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

Related Products


Why BioAssay Systems?

Expert Technical Support
Technical support provided by the very scientists that develop the assays.

Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market

Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.

Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.


BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers