QuantiFluo™ Caspase-3 Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of the apoptosis target caspase-3 activity and HTS screen for apoptosis inducers and inhibitors.

Key Features
Safe. Non-radioactive assay.

Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
FL400/490nm

Samples
Cell and tissue lysate

Species
All

Procedure
60 min

Size
100 tests

Shelf Life
12 months

More Details
Caspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis. Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3 is key biomarker in the assessment of apoptosis and in understanding mechanism of apoptosis induction. BioAssay Systems Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product.The fluorescence intensity (λex/em = 400/490nm) is proportional to the caspase-3 activity.
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QuantiFluo™ Caspase-3 Assay Kit
Catalog No: DCS3-100
Price: $209    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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