EnzyChrom™ Aspartate Transaminase Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity.

Key Features
Sensitive. Linear detection range: 2-100 U/L.

Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min.

Method
OD340nm

Samples
Serum, plasma etc

Species
All

Procedure
10 min

Size
100 tests

Detection Limit
2 U/L

Shelf Life
6 months

More Details
Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease and trauma. Simple, direct and automation-ready procedures for measuring AST activity find wide applications in research and drug discovery. BioAssay Systems AST activity assay is based on the quantification of oxaloacetate produced by AST. In this assay, oxaloacetate and NADH are converted to malate and NAD by the enzyme malate dehydrogenase. The decrease in NADH absorbance at 340 nm is proportionate to AST activity.

1. I am not quite clear about the factor 388 used in the calculation. How is path length correction for 220 µl of sample volume in the 96 well done? Is the concentration of the NADH used 10 mM?



The concentration of the NADH solution is 10 mM. The concentration of NADH in the final reaction volume (V = 220 μL) is calculated by accounting for the dilution steps when preparing the working reagent, i.e. 4 μL/206μL in the first step and 200 mL/220 mL in the second step. This is the final initial concentration of NADH in the reaction.

To calculate the activity of the sample, i.e. µmoles NADH consumed / (L x min), one has to divide by 5 min incubation time and multiply by the dilution factor of the sample which is 11.

Multiplying all these constant values together equals 388.

There is no need for a path length correction, because the sample and the standard wells contain the same volume.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Li, Yajuan, et al (2019). PTEN-induced partial epithelial-mesenchymal transition drives diabetic kidney disease. The Journal of clinical investigation 129.3. Assay: Aspartate transaminase in mice serum.

Hay, Charles E., et al (2018). Development and testing of AAV-delivered single-chain variable fragments for the treatment of methamphetamine abuse. PloS one 13.6: e0200060. Assay: Aspartate transaminase in mice serum.

Kamerkar, Sushrut, et al (2017). Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer. Nature 546.7659: 498. Assay: Aspartate transaminase in mice plasma.

Kim, Soo Hyun, et al (2017). Ezetimibe ameliorates steatohepatitis via AMP activated protein kinase-TFEB-mediated activation of autophagy and NLRP3 inflammasome inhibition. Autophagy 13.10: 1767-1781. Assay: Aspartate transaminase in mice serum.

Sharabi, K et al (2017). Selective chemical inhibition of PGC-1alpha gluconeogenic activity ameliorates type 2 diabetes. Cell 169.1: 148-160. Assay: Aspartate transaminase in mice serum.

Wardah, J. Rahmahani, and T. Sopandi (2017). Effect of Phyllanthus buxifolius Leaf as a Feed Supplement on Liver Function and Haematological Response of Quail (Coturnix coturnix japonica) Challenged with Infectious Newcastle Disease Virus. International Journal of Poultry Science 16: 354-363. Assay: Aspartate transaminase in quail serum.

Weis, Sebastian, et al (2017). Metabolic adaptation establishes disease tolerance to sepsis. Cell 169.7: 1263-1275. Assay: Aspartate transaminase in mice plasma.

Seemann, Semjon, and Amelie Lupp (2016). Administration of AMD3100 in endotoxemia is associated with pro-inflammatory, pro-oxidative, and pro-apoptotic effects in vivo. Journal of biomedical science 23.1: 68. Assay: Aspartate transaminase in mouse serum.

Laura, BP et al. (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture 454: 8-18. Assay: Aspartate transaminase in fish plasma.

Seemann, S and A Lupp (2015). Administration of a CXCL12 Analog in Endotoxemia Is Associated with Anti-Inflammatory, Anti-Oxidative and Cytoprotective Effects In Vivo. PLoS One 10(9): e0138389. Assay: Aspartate transaminase in mouse serum.

Jiang, S., et al (2014). Effect of perches on liver health of hens. Poultry science, 93(7), 1618-1622. Assay: Aspartate transaminase in hen serum.

Vives, V et al (2014). Pharmacological inhibition of Dock5 prevents osteolysis by affecting osteoclast podosome organization while preserving bone formation. Nature Communications 6: 6128. Assay: Aspartate transaminase in mouse serum.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyChrom™ Aspartate Transaminase Assay Kit
Catalog No: EASTR-100
Price: $299    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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