EnzyChrom™ Ascorbic Acid Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.

Key Features
Use 20 μL samples. Linear detection range: colorimetric assay 6 to 1000 μM, fluorimetric assay 1 to 100 μM ascorbic acid.

Method
OD570nm, or FL530/585nm

Samples
Serum, plasma, urine, saliva, milk, tissue, and cell culture

Species
All

Procedure
10 min

Size
100 tests

Detection Limit
OD, FL: 6, 1 μM

Shelf Life
6 months

More Details
Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. BioAssay Systems’ method provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.

1. We would like to measure ascorbic acid in liver homogenates, which are in 0.25M Sucrose. We want to confirm the sucrose will interfere with the assay?



We have not tested the compatibility of sucrose with this assay. Based on the mechanism of the assay we see no reason why sucrose would interfere and believe the assay will work just fine.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Trezzi, JP et al (2016). LacaScore: a novel plasma sample quality control tool based on ascorbic acid and lactic acid levels. Metabolomics 12: 96. Assay: Plasma in Humans (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ Ascorbic Acid Assay Kit
Catalog No: EASC-100
Price: $319    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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