QuantiChrom™ Arginase Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of arginase activity and screen for its inhibitors.

Key Features
Sensitive and accurate. Detection limit: 0.3 U/L for 2 hr arginase reaction in 96-well assay format.

Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate followed by the addition of the coloring reagent. Can be readily automated as a high-throughput assay for thousands of samples per day.

Method
OD430nm

Samples
Enzyme preparations, serum, plasma, tissue culture etc

Species
All

Procedure
2 hrs

Size
100 tests

Detection Limit
1 U/L (0.3 U/L for 2 hr reaction)

Shelf Life
12 months

More Details
ARGINASE (L-arginine ureohydrolase EC 3.5.3.1) is present in mammals and plants. In humans, arginase is expressed predominantly in the liver, and to lesser degrees in breast, kidney, testes, salivary glands, epidermis and erythrocytes. Arginase catalyzes the conversion of arginine to ornithine and urea, completing the last step in the urea cycle. Arginase activity is a key diagnostic indicator. Increased levels of arginase activity in blood have been associated with liver damage. Hyperargininemia due to arginase deficiency is an inherited autosomal recessive disease. Simple, direct and automation-ready procedures for measuring arginase activity in biological samples are highly desirable in Research and Drug Discovery. BioAssay Systems arginase assay kit provides a sensitive and convenient method for arginase activity determination. The method utilizes a chromogen that forms a colored complex specifically with urea produced in the arginase reaction. The intensity of the color is directly proportional to the arginase activity in the sample.

1. What are the best samples, serum or plasma? And with or without heparin?



Wash 5-10 x105 cells and resuspend cell pellet in 100 μL lysis buffer containing 0.4% Triton X-100, 25 mM Tris-HCl (pH 7.5) and 1 mM MnSO4 (10 μL of the provided Mn Solution). Optionally, activate arginase by heating for 10 min at 55-60°C. For serum samples we found the heating step unnecessary, but we have not tested cell or tissue samples. Centrifuge for 2 minutes at 14,000 rpm on a table centrifuge and use clear supernatant in arginase assay.

2. I see in your protocol that you don’t heat the sample what is normally done in the literature. Is you protocol different? Should I heat my samples to activate the enzyme?



We tried to heat the enzyme (purified enzyme and serum samples) in the assay buffer and found no significant difference as compared to no heating. Therefore our protocol does not include the heating step.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Zhao. J et al (2016). Botanical Drug Puerarin Attenuates 6-Hydroxydopamine(6-OHDA)-Induced Neurotoxicity via UpregulatingMitochondrial Enzyme Arginase-2. Mol Neurobiol. 53(4):2200-11. Assay: Arginase in rat pheochromocytoma cells.

Johnson, FK et al (2015). Arginase promotes endothelial dysfunction and hypertension in obese rats. Obesity (Silver Spring). 23(2):383-90. Assay: Arginase in obese and lean zucker rats plasma.

Csoka, B et al (2014). A2B Adenosine Receptors Prevent Insulin Resistance by Inhibiting Adipose Tissue Inflammation via Maintaining Alternative Macrophage Activation. Diabetes. 63(3):850-66. Assay: Arginase in rat adipose tissue.

Nishio, H et al (2014). Immunosuppression through constitutively activated NF-kB signalling in human ovarian cancer and its reversal by an NF-kB inhibitor. Br J Cancer. 110(12):2965-74. Assay: Arginase in human epithelial cells.

Rodriguez, MP et al (2014). Role of Metabolic Environment on Nitric Oxide Mediated Inhibition of Neointimal Hyperplasia in Type 1 and Type 2 Diabetes. Nitric Oxide. 36:67-75. Assay: Arginase in lean zucker rats plasma.

Anderson JT, et al (2011). Elevated levels of NO are localized to distal airways in asthma. Free Radic Biol Med. 50(11):1679-88. Assay: Arginase in human bronchial fluids.

Hegde S, et al (2011). human NKT cells direct the differentiation of myeloid APCs that regulate T cell responses via expression of programmed cell death ligands. J Autoimmun. 37(1):28-38. Assay: Arginase in human cells.

Kennedy DJ, et al (2011). A CD36-dependent pathway enhances macrophage and adipose tissue inflammation and impairs insulin signalling. Cardiovasc Res. 89(3):604-13. Assay: Arginase in mouse plasma.

Lechner MG, et al (2011). Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines. J Transl Med. 9:90. Assay: Arginase in human cells.

Ma, G et al (2011). Paired immunoglobin-like receptor-B regulates the suppressive function and fate of myeloid-derived suppressor cells. Immunity 34(3):385-95. Assay: Arginase in mouse macrophages.

Riley E, et al (2011). Inhibition profile of Leishmania mexicana arginase reveals differences with human arginase I. Int J Parasitol. 41(5):545-52. Assay: Arginase in human protein extracts.

Eruslanov E, et al (2010). Pivotal Advance: Tumor-mediated induction of myeloid-derived suppressor cells and M2-polarized macrophages by altering intracellular PGE2 catabolism in myeloid cells. J Leukoc Biol.88(5):839-48. Assay: Arginase in macrophages.

Feola DJ, et al (2010). Azithromycin alters macrophage phenotype and pulmonary compartmentalization during lung infection with Pseudomonas. Antimicrob Agents Chemother. 54(6):2437-47. Assay: Arginase in mouse cell lysates.

Sripadi P, et al (2010). Direct detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry. PLoS One 5(9):e12590. Assay: Arginase in human cell line.

Wang D, et al (2010). Elevated mitochondrial reactive oxygen species generation affects the immune response via hypoxia-inducible factor-1alpha in long-lived Mclk1+/- mouse mutants. J Immunol. 184(2):582-90. Assay: Arginase in mouse tissue.

Capuano G, et al (2009). Modulators of arginine metabolism support cancer immunosurveillance. BMC Immunol. 10:1. Assay: Arginase in mouse CD11b+ cells.

Eruslanov E, et al (2009). Altered expression of 15-hydroxyprostaglandin dehydrogenase in tumor-infiltrated CD11b myeloid cells: a mechanism for immune evasion in cancer. J Immunol. 182(12):7548-57. Assay: Arginase in mouse cell lysates.

Weiss, JM et al (2009). Successful immunotherapy with IL-2/anti-CD40 induces the chemokine-mediated mitigation of an immunosuppressive tumor microenvironment. PNAS 106(46):19455-60. Assay: Arginase in mouse tumor tissue.

Bao B, et al (2008). Zinc supplementation decreases oxidative stress, incidence of infection, and generation of inflammatory cytokines in sickle cell disease patients. Transl Res. 152(2):67-80. Assay: Arginase in human tissue.

Moertel L, et al (2008). Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum. Parasitology 135(5):575-83. Assay: Arginase in snail protein extracts.

Murphy BS, et al (2008). Azithromycin alters macrophage phenotype. J Antimicrob Chemother. 61(3):554-60. Assay: Arginase in human cells.

Schwacha MG, et al (2008). Burn injury-induced alterations in wound inflammation and healing are associated with suppressed hypoxia inducible factor-1alpha expression. Mol Med. 14(9-10):628-33. Assay: Arginase in mouse wound homogenates.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
Buy Now!!!
QuantiChrom™ Arginase Assay Kit
Catalog No: DARG-100
Price: $209    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: 4, -20°C

Related Products


Why BioAssay Systems?

Expert Technical Support
Technical support provided by the very scientists that develop the assays.

Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market

Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.

Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.


BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers