EnzyChrom™ α-Amylase Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of α-amylase activity.

Key Features
Sensitive and accurate. Linear detection range 0.3 to 50 U/L α-amylase in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, incubation for 15 min, followed by the detection reagent and a 20-min incubation and reading the optical density at 585 nm.

Method
OD585nm

Samples
Blood, saliva, urine, agriculture etc

Species
All

Procedure
40 min

Size
100 tests

Detection Limit
0.3 U/L

Shelf Life
6 months

More Details
AMYLASE belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure. Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. BioAssay Systems’ EnzyChrom™ a-amylase assay method involves two steps: (1). α-amylase in the sample hydrolyzes starch and the product is rapidly converted to glucose by α-glucosidase and hydrogen peroxide by glucose oxidase; (2). hydrogen peroxide concentration is determined with a colorimetric reagent.

11. Does glucose interfere with the assay?



The glucose content of the sample will add a significant background and I would recommend removing it from the sample:

- For samples known to contain glucose, use a membrane filter (e.g.

Microcon YM-10 from Millipore) to remove glucose: load 50 μL sample in a Microcon YM-10 (10 kDa cutoff) and add 500 μL Assay Buffer.

- Centrifuge at 14000 rpm for 30 min, check level of sample, ideally the sample level will be less than 50 μL. Add 500 μL Assay Buffer and repeat the centrifugation. Measure final sample volume with a pipetman and calculate dilution factor n = final sample volume/50 μL.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Mohannad N et al (2014). Age-related changes in salivary biomarkers. Journal of Dental Sciences. 9(1): 85-90. Assay: a-Amylase in Human Saliva.

2. S. B. Lee et al (2013). Effect of Oral Administration of Intact Casein on Gastrointestinal Hormone Secretion and Pancreatic ?-Amylase Activity in Korean Native Steer. Asian-Australasian Journal of Animal Sciences (AJAS). 26(5): 654-660. Assay: a-Amylase in steers (cow) pancreas (duodenal fluid).

3. Lee, Kyung-Hoon, et al.(2014). Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats. J. Animal Sci. Techn. 56.1 : 6. Assay: a-Amylase in Rat plasma.

4. Abu Khadra, KM et al (2014)/ Antioxidant Profile of Saliva among Young Men Using Mobile Phones. Jordan Journal of Biological Sciences. 7(4):275-280.

5.Han, Mee-Jung, and Seung Hwan Lee (2015). An efficient bacterial surface display system based on a novel outer membrane anchoring element from the Escherichia coli protein YiaT. FEMS Microbiol. Lett. 362.1: 1-7. Assay: a-Amylase in Bacillus subtilis (bacteria) cells.

6. "Lee, KH et al (2014). Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats. Journal of Animal Science and Technology. 56:6 Assay: Pancreas Tissue in Sprague-Dawley rats (Pubmed).

To find more recent publications, please click here.
Buy Now!!!
EnzyChrom™ α-Amylase Assay Kit
Catalog No: ECAM-100
Price: $369    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: 4, -20°C



BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers