EnzyChrom™ Ammonia/Ammonium Assay Kit
ProtocolSDS


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Application
For quantitative determination of ammonia/ammonium concentration.

Key Features
High sensitivity and wide linear range. Use 20 μL sample. Linear detection range 24 to 1000 μM ammonia.

Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ammonia within 30 minutes.

Method
OD340nm, or FL360/450nm

Samples
Serum, plasma, urine, saliva, cell culture etc

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
24 μM

Shelf Life
6 months

More Details
Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase). Simple, direct and automation-ready procedures for measuring NH3 are popular in research and drug discovery. BioAssay Systems ammonia assay is designed to directly measure NH3 and NH4+. In this assay, NADH is converted to NAD+ in the presence of NH3, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at λex/em = 360/450 nm is directly proportionate to the NH3 concentration in the sample.

1. Does glutamate in the sample interfere with the assay?



High glutamate concentrations (> 2 mM) will interfere with the assay. For example, a typical concentration of glutamate in cell culture medium is ~ 0.15 mM.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Rauner, G et al (2013). Development of Foreign Mammary Epithelial Morphology in the Stroma of Immunodeficient Mice. PloS one 8.6: e68637. Assay: Ammonia in Mouse Cell Culture Media (Pubmed).

2. Nunez, EGF et al (2014). Influence of aeration-homogenization system in stirred tank bioreactors, dissolved oxygen concentration and pH control mode on BHK-21 cell growth and metabolism. Cytotechnology 1-13. Assay: Ammonia in Hamster Kidney Cell Line (Pubmed).

3. Wang, L et al (2015). CtBP maintains cancer cell growth and metabolic homeostasis via regulating SIRT4. Cell Death and Disease 6: e1620. Assay: Tumor Tissue in Mice (Pubmed).

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
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EnzyChrom™ Ammonia/Ammonium Assay Kit
Catalog No: ENH3-100
Price: $259    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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