QuantiFluo™ Alkaline Phosphatase Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For quantitative determination of alkaline phosphatase (ALP) activity using stable 4-methylumbelliferyl phosphate substrate.

Key Features
High sensitivity and wide linear range. Use 10 μL sample. Detection limit of 0.02 U/L (20 min reaction).

Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 20 minutes.

Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

Method
FL360/450nm

Samples
Serum, plasma etc

Species
All

Procedure
20 min

Size
100 tests

Detection Limit
0.02 U/L

Shelf Life
2 years

More Details
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery. This improved method utilizes 4-methylumbelliferyl phosphate that is hydrolyzed by ALP into a highly fluorescent product 4-methylumbelliferone. The rate of the fluorescence increase is directly proportional to the enzyme activity.
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1. Laura, BP et al. (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture. 454 (2016) 8–18. Assay: Plasma in Fish.

2. Maryam, RR et al. (2015). The role of dentin matrix protein 1 (DMP1) in regulation of osteogenic differentiation of rat dental follicle stem cells (DFSCs). Arch Oral Biol. 60(4): 546–556. Assay: Cells in Rats. (Pubmed).

To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

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Please inquire via email or call 1-510-782-9988 x 2.
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QuantiFluo™ Alkaline Phosphatase Assay Kit
Catalog No: QFAP-100
Price: $169    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: RT
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

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