QuantiChrom™ BCG Albumin Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of albumin.

Key Features
Sensitive and accurate. Use as little as 5 μL samples. Detection range 0.01 - 5 g/dL (1.5 - 750 μM) albumin in 96-well plate assay.

Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.

No interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.

Method
OD620nm (BCG)

Samples
Serum, plasma, urine, biological preparations

Species
All

Procedure
5 min

Size
250 tests

Detection Limit
0.01 g/dL

Shelf Life
12 months

More Details
Albumin is the most abundant plasma protein in human. It accounts for about 60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure, binding of key substances such as long-chain fatty acids, bile acids, bilirubin, haematin, calcium and magnesium. It has anti-oxidant and anticoagulant effects, and also acts as a carrier for nutritional factors and drugs, as an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition and protein-losing enteropathies. High levels are associated with dehydration. Simple, direct and automation-ready procedures for measuring albumin concentration in biological samples are becoming popular in Research and Drug Discovery. BioAssay Systems’ BCG albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol green that forms a colored complex specifically with albumin. The intensity of the color, measured at 620nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

1. Your company sells two different kit (DIAG-250 and DIAP-250). What are the differences between them exactly? Is it just the wavelength of detection?



The two kits use different albumin specific dyes and hence the major difference lies in the sensitivity and wavelengths. Both assays have advantages and disadvantages. DIAG-250 (QuantiChrom BCG Albumin Assay Kit) uses bromcresol green, a dye that is more (about 2-fold) sensitive than bromcresol purple used in the BCP albumin assay. BCG is not absolutely specific for albumin, however, it also reacts with α1- and α2-globulins, and adds a positive bias. The BCP method, although more specific, underestimates albumin in certain samples, e.g. from patients undergoing hemodialysis. If your samples are sera or plasma, which have high albumin concentrations, I would suggest using the DIAP-250 assay kit (detection wavelength 610 nm). If the albumin concentration is low, DIAG-250 is the best choice (detection wavelength 620 nm).

2. Pooled plasma samples (EDTA) show high albumin concentrations with the bromcresol purple assay? What could be interfering with the assay?



There should be no significant differences between serum samples and plasma samples. Turbid plasma samples (fibrinogen) can cause high albumin readings. This can be adjusted for by blanking samples at OD 690 nm. BCP does not absorb at 690 nm, whereas the absorbance caused by turbididty is the same at 610 nm and 690 nm.

3. Since the concentration that I am measuring is very low, would it be reasonable to dilute the standard curve down to lower level so my sample is still within the curve?



Yes, as long as the standard curve is within the limits of the dynamic range, but this will not increase the sensitivity of the assay. To increase the sensitivity add a larger volume of sample and standard. For example, you could add 20 μL sample and 20 μL of diluted standards (instead of starting at 5 g/dL as shown in the standard protocol, start at 1 g/dL).

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Kain, R et al (2008). Molecular mimicry in pauci-immune focal necrotizing glomerulonephritis. Nat Med. 14(10):1088-96. Assay: Albumin in Human albuminuria (Pubmed).

2. Lee, RH et al (2006) Multipotent stromal cells from human marrow home to and promote repair of pancreatic islets and renal glomeruli in diabetic NOD_scid mice. PNAS 103 (46): 17438–17443. Assay: Albumin in Mouse urine (Pubmed).

3. Helsby, NA et al (2008). CYP2C19 pharmacogenetics in advanced cancer: compromised function independent of genotype. Br J Cancer. 99(8):1251-5. Assay: Albumin in Human serum (Pubmed).

4. Doi, M et al (2011). Association between calcium in cord blood and newborn size in Bangladesh. Br J Nutr. 2011 Jun 1:1-10. Assay: Albumin in Human blood (Pubmed).

5. Zhang, Q et al (2006). Serum and mucosal antibody responses to pneumococcal protein antigens in children: relationships with carriage status. Eur J Immunol.36(1):46-57. Assay: Albumin in Human saliva, serum (Pubmed).

6. Cosgrove, D et al (2008). Integrin alpha1β1 Regulates Matrix Metalloproteinases via P38 Mitogen-Activated Protein Kinase in Mesangial Cells. Implications for Alport Syndrome. Am. J. Pathology 172:761-773. Assay: Albumin in Mouse urine (Pubmed).

7. Zhang, G et al (2011). Nicotinic acetylcholine receptor α1 promotes calpain-1 activation and macrophage inflammation in hypercholesterolemic nephropathy. Lab Invest. 91(1):106-23. Assay: Albumin in Mouse urine (Pubmed).

8. Euhus, DM et al (2011). Tamoxifen Downregulates Ets-oncogene Family Members ETV4 and ETV5 in Benign Breast Tissue: Implications for Durable Risk Reduction. Cancer Prev Res (Phila). 2011 Jul 21. Assay: Albumin in Human blood (Pubmed).

9. Wang, S et al (2006). Renal bone morphogenetic protein-7 protects against diabetic nephropathy. J Am Soc Nephrol. 17(9):2504-12. Assay: Albumin in Mouse serum (Pubmed).

10. Rebecca, R (2006). Associations of histories of depression and PMDD diagnosis with allopregnanolone concentrations following the oral administration of micronized progesterone sychoneuroendocrinology 31(10):1208-1219. Assay: Albumin in Human serum (Pubmed).

11. Sullivan, JP et al (2008). Convection and hemoglobin-based oxygen carrier enhanced oxygen transport in a hepatic hollow fiber bioreactor. Artif Cells Blood Substit Immobil Biotechnol.36(4):386-402. Assay: Albumin in Human hepatocyte (Pubmed).

12. Osborn, TM et al (2008). Decreased levels of the gelsolin plasma isoform in patients with rheumatoid arthritis. Arthritis Res Ther. 10(5):R117. Assay: Albumin in Human plasma, synovial fluid (Pubmed).

13. Roberts, DJ et al (2009). Effect of acute inflammatory brain injury on accumulation of morphine and morphine 3- and 6-glucuronide in the human brain. Crit Care Med. 37(10):2767-74. Assay: Albumin in Human plasma, cerebrospinal fluid (Pubmed).

14. Irani, K et al (2010). Mechanical dissociation of swine liver to produce organoid units for tissue engineering and in vitro disease modeling. Artif Organs. 34(1):75-8. Assay: Albumin in Pig hepatocyte (Pubmed).

To find more recent publications, please click here. To find more recent publications, please click here.
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QuantiChrom™ BCG Albumin Assay Kit
Catalog No: DIAG-250
Price: $269    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: RT
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: 4, -20°C



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