EnzyChrom™ Alanine Transaminase Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of alanine transaminase activity.

Key Features
Sensitive. Linear detection range: 2-100 U/L.

Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min.

Method
OD340nm

Samples
Serum, plasma etc

Species
All

Procedure
10 min

Size
100 tests

Detection Limit
2 U/L

Shelf Life
6 months

More Details
Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage. Simple, direct and automation-ready procedures for measuring ALT activity find wide applications in research and drug discovery. BioAssay Systems’ ALT activity assay is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate dehydrogenase (LDH). The decrease in NADH absorbance at 340 nm is proportional to ALT activity.

1. In regards to the ALT Assay Kit (EALT-100), do you guys sell a positive control?



We do not sell a positive control for this assay. Purified ALT is available from companies such as Sigma. Alternatively, serum samples could be used, for instance FBS (not heat inactivated).

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Mani V et al (2012) Effects of the Total Alkaloidal Extract of Murraya koenigii Leaf on Oxidative Stress and Cholinergic Transmission in Aged Mice. Phytotherapy Research . Assay: Alanine Transaminase in mice serum (Pubmed).

2. Rattan R et al (2011). Metformin suppresses ovarian cancer growth and metastasis with enhancement of cisplatin cytotoxicity in vivo. Neoplasia 13(5):483-491. Assay: Alanine Transaminase in Mouse Plasma (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ Alanine Transaminase Assay Kit
Catalog No: EALT-100
Price: $259    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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