EnzyChrom™ Adipolysis Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.

Key Features
Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.92 to 100 μg/mL (10 to 1000 μM) glycerol for colorimetric assays and 0.2 to 5 μg/mL for fluorimetric assays.

Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.

Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates.

Method
OD570nm, or FL530/585nm

Samples
Cell culture media

Species
All

Procedure
20 min

Size
200 tests

Detection Limit
0.92 μg/mL

Shelf Life
12 months

More Details
Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. BioAssay Systems’ adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm is directly proportional to glycerol concentration in the sample.

1. I collected the medium from the culture wells. Do I need to now centifuge the medium and collect the supernatant or do I use the medium itself for the assay?



You can assay the cell culture medium directly. However, if it contains cellular debris you should first centrifuge it and then use the clear supernatant in the assay.

2. What is the principle of the assay?



In the process of adipolysis triglycerides are hydrolyzed by the cellular lipases into glycerol and free fatty acids. The assay measures glycerol, the end product of adipolysis, in a coupled three step enzyme reaction:

1) Glycerol kinase converts glycerol and ATP to glycerol phosphate and ADP.

2) Glycerol phosphate oxidase converts glycerol phosphate and O2 to glycerone phosphate and H2O2

3) HRP converts ADHP and H2O2 to resorufin and water.

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Li R et al (2012). Neuropeptide Y potentiates beta-adrenergic stimulation of lipolysis in 3T3-L1 adipocytes. Regul Pept. 178(1-3):16-20. Assay: Adipolysis in Mouse 3T3-L1 adipocytes (Pubmed).

2. Huttala, O et al (2016). Differentiation of human adipose stromal cells in vitro into insulin-sensitive adipocytes. Cell and Tissue Research 1-12. Assay: Cells in Humans (Pubmed).

3. Kim, HK et al (2016). An apolipoprotein B100 mimotope prevents obesity in mice. Clinical Science 130(2): 105-16. Assay: Adipocytes in Mice (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ Adipolysis Assay Kit
Catalog No: EAPL-200
Price: $299    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: 4, -20°C



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