EnzyChrom™ Acetate Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism.

Key Features
Use as little as 10 μL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays.

Method
OD570nm, or FL530/585nm

Samples
Serum, plasma, food, agriculture and environment

Species
All

Procedure
30 min

Size
100 tests

Detection Limit
0.13 mM

Shelf Life
6 months

More Details
Acetate is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and extensively used in food, dyes, paints, glue and synthetic fibres. BioAssay Systems’ assay uses enzyme-coupled reactions to form a colored, fluorescent product. The color absorbance at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the acetate concentration in the sample.
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For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Garrido D et al (2013). Utilization of galactooligosaccharides by Bifidobacterium longum subsp. infantis isolates. Food Microbiol. 33(2):262-70. Assay: Acetate in Microbe cell (Pubmed).

2. Cuervo-Soto, LI et al (2015). Identification of a novel carbohydrate esterase from Bjerkandera adusta: Structural and function predictions through bioinformatics analysis and molecular modeling. Proteins: Structure, Function, and Bioinformatics 83(3): 533-546. Assay: Acetate in Microbe culture (Pubmed).

To find more recent publications, please click here.
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EnzyChrom™ Acetate Assay Kit
Catalog No: EOAC-100
Price: $319    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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