EnzyFluo™ Acetaldehyde Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
•  Acetaldehyde determination in biological and food/beverage samples.

Key Features
•  Fast and sensitive. Linear detection range (50µL sample): 0.5 µM to 60 µM acetaldehyde in 96-well plate assay.

•  High-throughput. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.

•  Convenient. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method
•  FL530/585nm

Samples
•  Biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice).

Species
•  All

Procedure
•  30 min

Size
•  100 tests

Detection Limit
•  0.5 µM 

Shelf Life
•  6 months

More Details
•  ACETALDEHYDE (CH3CHO) is one of the most widely occurring aldehydes in nature and is commonly used in industry. Acetaldehyde, a metabolic byproduct of ethanol in the liver, is toxic to the human body and is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing often referred to as “Asian flush syndrome.” Build up of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine. BioAssay Systems' colorimetric acetaldehyde assay kit is based on aldehyde dehydrogenase catalyzed oxidation of acetaldehyde, in which the formed NADH reduces a formazan reagent. The intensity of the product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.

1. How specific is this assay for acetaldehyde?



The assay mechanism uses the enzyme aldehyde dehydrogenase to quantify acetaldehyde in the sample. The enzyme will also oxidize other aldehydes in the sample (i.e. propionaldehyde). Therefore, the assay will not distinguish between acetaldehyde and other aldehydes present in the sample. Consideration should be taken if high levels of other aldehydes are expected in the sample being tested.

2. How long are the diluted standards good for?



Acetaldehyde is a rather volatile chemical, so proper storage of the standard is vital. Be sure to keep the Standard and standard dilutions on ice or refrigerated with the tube caps closed during assay procedure. For the colorimetric assay, the standards (4 mM, 2.4 mM, 1.2 mM, and 0 mM) should be stable for the day they are made. For the fluorimetric assay, we recommend remaking the standards (60 µM, 36 µM, 18 µM, and 0 µM) fresh from the 4 mM Acetaldehyde for each assay.

3. What temperature do I run the assay at?



The assay may be run at room temperature (25°C) or 37°C.



For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
No citations for this new product. Please check back later.
You may click here to check if citations are available, but are not listed here yet.
Buy Now!!!
EnzyFluo™ Acetaldehyde Assay Kit
Catalog No: FACT-100
Price: $229    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment:
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers