EnzyLight™ ATP Assay Kit
Datasheet PDF  MSDS/Bulk Order


Application
For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism.

Key Features
Safe. Non-radioactive assay.

Sensitive and accurate. As low as 0.02 μM ATP or a single cell can be quantified.

Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.

Robust and amenable to HTS: Z’ factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Method
Luminescence

Samples
Cells etc

Species
All

Procedure
10 min

Size
100 tests

Detection Limit
0.1 μM

Shelf Life
12 months

More Details
Adenosine 5’-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. BioAssay Systems’ EnzyLight™ ATP Assay Kit provides a rapid method to measure intracellular ATP. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

1. Can this kit be used to adhesive cells?



This kit is suitable for adherent cells. The assay buffer contains 0.5 % Triton X-100 that will efficiently lyse the cells.

2. Is the lysis buffer in the kit suitable for Bradford protein assay?



The final Triton X-100 concentration in the lysate will be around 0.25 % which would interfere with the Bradford assay. Therefore, I would recommend diluting the lysate further. The commonly used BCA assay is not compatible, because of the high amount of mercaptoethanol in the assay buffer (70 mM).

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
1. Chandak PG, et al (2010). Efficient phagocytosis requires triacylglycerol hydrolysis by adipose triglyceride lipase. J Biol Chem.285(26):20192-201. Assay: cells in mouse (Pubmed).

2. Aflaki E, et al (2011). Triacylglycerol accumulation activates the mitochondrial apoptosis pathway in macrophages. J Biol Chem. 286(9):7418-28. Assay: macrophage in mouse (Pubmed).

3. Schwarzer C, et al (2008). Oxidative stress caused by pyocyanin impairs CFTR Cl(-) transport in human bronchial epithelial cells. Free Radic Biol Med. 45(12):1653-62. Assay: cell in human (Pubmed).

4. Belleannee C, et al (2010). Role of purinergic signaling pathways in V-ATPase recruitment to apical membrane of acidifying epididymal clear cells. Am J Physiol Cell Physiol. 298(4):C817-30. Assay: tube in rat (Pubmed).

5. Dvoriantchikova G, et al (2010). Liposome-delivered ATP effectively protects the retina against ischemia-reperfusion injury. Mol Vis.16:2882-90. Assay: retina in mouse (Pubmed).

6. Khuda-Buksh, AR. et al. (2011). Analysis of the capability fo ultra-highly diluted glucose to increase glucose uptake in arsenite-streesed bacteria Escherichia coli. J, Chin. Integrative Medicine 9(8): 901-912. Assay: cell lysate in bacteria (Pubmed).

7. Sugimoto S, et al (2009). Apyrase treatment prevents ischemia-reperfusion injury in rat lung isografts. J Thorac Cardiovasc Surg. 138(3):752-9. Assay: lung tissue in rat (Pubmed).

8. Ponnusamy M, et al (2011). P2X7 receptors mediate deleterious renal epithelial-fibroblast cross talk. Am J Physiol Renal Physiol. 300(1):F62-70. Assay: fibroblast in rat (Pubmed).

9. Choi EM, Lee YS (2011). Protective effect of apocynin on antimycin A-induced cell damage in osteoblastic MC3T3-E1 cells. J Appl Toxicol. 2011 May 2. doi: 10.1002/jat.1689. Assay: cell in mouse (Pubmed).

10. Methods for treatment of oncological disorders using an epimetabolic shifter (coenzyme q10). US Patent Appl. 20110027247. Assay: cell in mouse.

11. Methods for treatment of metabolic disorders using epimetabolic shifters, mutlidimensional intracellular molecules, or environmental influencers. US Patent Appl. 20110020312. Assay: cell, environmental in human.


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EnzyLight™ ATP Assay Kit
Catalog No: EATP-100
Price: $269    Qty:
For orders of 10 or more kits, please contact us at 1-510-782-9988 x 1 for best pricing.

Shipping: On Ice
Shipment: Same Day for order by 2pm PST
Delivery: 1-2 days (US), 3-6 days (Intl);
Storage: -20°C



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