EnzyLight™ ATP Assay Kit
Protocol SDS  Printed Protocols and SDSs are not provided with kits.

Application
For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism.

Key Features
Safe. Non-radioactive assay.

Sensitive and accurate. As low as 0.02 μM ATP or a single cell can be quantified.

Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.

Robust and amenable to HTS: Z factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Method
Luminescence

Samples
Cells etc

Species
All

Procedure
10 min

Size
100 tests

Detection Limit
0.1 μM

Shelf Life
12 months

More Details
Adenosine 5’-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as ?energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. BioAssay Systems' EnzyLight™ ATP Assay Kit provides a rapid method to measure intracellular ATP. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

1. Can this kit be used to adhesive cells?



This kit is suitable for adherent cells. The assay buffer contains 0.5 % Triton X-100 that will efficiently lyse the cells.

2. Is the lysis buffer in the kit suitable for Bradford protein assay?



The final Triton X-100 concentration in the lysate will be around 0.25 % which would interfere with the Bradford assay. Therefore, I would recommend diluting the lysate further. The commonly used BCA assay is not compatible, because of the high amount of mercaptoethanol in the assay buffer (70 mM).

For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions
Le, Jiamei, et al (2019). Regulation of microbiota-GLP1 axis by Sennoside A (SA) in diet-induced obese mice. Acta Pharmaceutica Sinica B. Assay: ATP in mice tissues.

Cai, J-G., et al (2018). Cytotoxicity of Malondialdehyde and Cytoprotective Effects of Taurine via Oxidative Stress and PGC-1alpha Signal Pathway in C2C12 Cells. Molecular Biology 52.4: 532-542. Assay: ATP in mouse cells.

Fan, Fengyan, et al (2018). Effects of red blood cell supernatants on hypoxia/reoxygenation injury in H9C2 cells. Intl.J. Clin. Exp. Med.11.4: 3612-3619. Assay: ATP in rat H9C2 cells.

Hashim, Mahira, et al (2018). Inhibition of SNAT5 induces incretin-responsive state from incretin-unresponsive state in pancreatic beta-cells: study of beta-cell spheroid clusters as a model. Diabetes 67.9: 1795-1806. Assay: ATP in mice pancreatic beta-cells.

Hoque, SA Masudul, et al (2018). Mitochondrial protein turnover is critical for granulosa cell proliferation and differentiation in antral follicles. Journal of the Endocrine Society 3.2: 324-339. Assay: ATP in mouse granulosa cell.

Ouyang, Pengfei, et al (2018). Increasing oxygen availability for improving poly (3-hydroxybutyrate) production by Halomonas. Metabolic engineering 45: 20-31. Assay: ATP in Halomonas bluephagenesis cells.

Pan, Jin-Xiu, et al (2018). APP promotes osteoblast survival and bone formation by regulating mitochondrial function and preventing oxidative stress. Cell death & disease 9.11: 1077. Assay: ATP in mice cells.

Shen, W., et al (2018). Mitochondria-mediated disturbance of fatty acid metabolism in proximal tubule epithelial cells leads to renal interstitial fibrosis. Eur Rev Med Pharmacol Sci 22.3: 810-819. Assay: ATP in mice serum.

Sun, Yongning, et al (2018). Restoration of GLP-1 secretion by Berberine is associated with protection of colon enterocytes from mitochondrial overheating in diet-induced obese mice. Nutrition & diabetes 8.1: 53. Assay: ATP in mice tissues.

Zhong, Hanhui, et al (2018). Propofol inhibits parthanatos via ROS-ER-calcium-mitochondria signal pathway in vivo and vitro. Cell death & disease 9.10: 932. Assay: ATP in mice cells.

Lin, Zou, et al (2017). Protective effect of alpha-lipoic acid against antimycin A cytotoxicity in MC3T3-E1 osteoblastic cells. Cell Stress and Chaperones 22.1: 5-13. Assay: ATP in mouse cells.

Rink, Cameron, et al (2017). Glutamate oxaloacetate transaminase enables anaplerotic refilling of TCA cycle intermediates in stroke-affected brain. The FASEB Journal 31.4: 1709-1718. Assay: ATP in mice brain tissues.

Aldonza, Mark Borris D., et al (2016). Multiplicity of acquired cross-resistance in paclitaxel-resistant cancer cells is associated with feedback control of TUBB3 via FOXO3a-mediated ABCB1 regulation. Oncotarget 7.23: 34395. Assay: ATP in human cells.

Joshi, A., et al (2016). Nuclear ULK1 promotes cell death in response to oxidative stress through PARP1. Cell death and differentiation 23.2: 216. Assay: ATP in murine cells.

Suh, Kwang Sik, Suk Chon, and Eun Mi Choi (2016). Luteolin alleviates methylglyoxal-induced cytotoxicity in osteoblastic MC3T3-E1 cells. Cytotechnology 68.6: 2539-2552. Assay: ATP in mouse cells.

Aflaki E, et al (2011). Triacylglycerol accumulation activates the mitochondrial apoptosis pathway in macrophages. J Biol Chem. 286(9):7418-28. Assay: ATP in mouse macrophage.

Choi EM, Lee YS (2011). Protective effect of apocynin on antimycin A-induced cell damage in osteoblastic MC3T3-E1 cells. J Appl Toxicol. 32(9):714-21. Assay: ATP in mouse cell.

Khuda-Buksh, AR. et al. (2011). Analysis of the capability fo ultra-highly diluted glucose to increase glucose uptake in arsenite-streesed bacteria Escherichia coli. J, Chin. Integrative Medicine 9(8): 901-912. Assay: ATP in bacteria cell lysate.

Khuda-Buksh, AR. et al. (2011). Analysis of the capability fo ultra-highly diluted glucose to increase glucose uptake in arsenite-streesed bacteria Escherichia coli. J, Chin. Integrative Medicine 9(8): 901-912. Assay: ATP in bacteria.

Methods for treatment of oncological disorders using an epimetabolic shifter (coenzyme q10). US Patent Appl. 20110027247. Assay: ATP in mouse cell.

Narain, NR, McCook, JP. (2011). Methods for treatment of oncological disorders using an epimetabolic shifter (coenzyme q10). US Patent Appl. 20110027247. Assay: ATP in human cell.

Ponnusamy M, et al (2011). P2X7 receptors mediate deleterious renal epithelial-fibroblast cross talk. Am J Physiol Renal Physiol. 300(1):F62-70. Assay: ATP in rat fibroblast.

Belleannee C, et al (2010). Role of purinergic signaling pathways in V-ATPase recruitment to apical membrane of acidifying epididymal clear cells. Am J Physiol Cell Physiol. 298(4):C817-30. Assay: ATP in rat tube.

Chandak PG, et al (2010). Efficient phagocytosis requires triacylglycerol hydrolysis by adipose triglyceride lipase. J Biol Chem.285(26):20192-201. Assay: ATP in mouse cells.

Dvoriantchikova G, et al (2010). Liposome-delivered ATP effectively protects the retina against ischemia-reperfusion injury. Mol Vis.16:2882-90. Assay: ATP in mouse retina.

Sugimoto S, et al (2009). Apyrase treatment prevents ischemia-reperfusion injury in rat lung isografts. J Thorac Cardiovasc Surg. 138(3):752-9. Assay: ATP in rat lung tissue.

Schwarzer C, et al (2008). Oxidative stress caused by pyocyanin impairs CFTR Cl(-) transport in human bronchial epithelial cells. Free Radic Biol Med. 45(12):1653-62. Assay: ATP in human cell.

To find more recent publications, please click here.
Please inquire or request assay service or call 1-510-782-9988 x 2.
Buy Now!!!
EnzyLight™ ATP Assay Kit
Catalog No: EATP-100
Price: $289    Qty:
For orders of 10 or more kits, please call 1-510-7829988x1 or email us for best pricing and/or bulk order.

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

Related Products


Why BioAssay Systems?

Expert Technical Support
Technical support provided by the very scientists that develop the assays.

Quality and User-friendly
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Competitive Prices
Because we develop and manufacture the products, our prices are lower than competitors on the market

Expansive Catalogue
With over 200 different products, acquire all your assay kit needs in one order.

Trusted Globally
Products used by clients worldwide with distributors in over 60 countries.


BioAssay Systems is committed to producing innovative, high-quality and cost-effective products
and to providing expert technical service to our valued customers